Table of Contents

1a. no spots...................go to 2
1b. spots........................go to 3

2a. metallic blue back............chum salmon
2b. no metallic blue back................sockeye salmon

3a. has adipose fin................go to 4
3b. no adipose fin..................go to 5

4a. flat body.............halibut
4b. round body........... northern pike

5a. light spots...............dolly
5b. dark spots...............go to 6

6a.no spots on the caudal fin............... arctic grayling
6b. spots on the caudal fin................go to 7

7a. spots on only the upper lobe of the caudal fin.............Silver Salmon
7b. spots on both lobes of the caudal fin................go to 8

8a. does not have spots on its belly..........go to 9
8b. has spots on its belly............rainbow trout

9a large ovular spots on its back and both lobes of caudal fin............Pink Salmon
9b circular spots on its back and on both lobes of caudal fin...........go to 10

10a black gum line...............King Salmon
10b white gum line ................ Steelhead

Cell Transport Lab

Focus Question
How can passive transport be demonstrated using a gelatin "cell" model?
Materials
Gelatin cell, beaker, 20 to 30 mm of ammonia, filter paper, rubber band, paper towel, timer, goggles, ruler.
Procedure
Photo_on_2011-09-19_at_14.22.jpg
Results
Time Observations
0 clear whitish tan squishy
2 bottom very pink rest looks pink
4 bottom part is darker pink
6 bottom is completely pink middle and top are actually clear
8 middle is becoming more pink
10 middle is pink top is becoming pink
dark pink 4 mm into gelatin

Analysis/Conclusions
1. Which cell transport process caused the gelatin "cell" to undergo changes without coming in direct contact with the ammonia? Be sure to include what evidence you have that this process occurred. Diffusion because the vapors (not water) from the ammonia entered the cell and the cell did not have protein channels because it was just gelatin. The diffusion reached 4 mm into the cell in 10 min,
2. What acts as a selectively permeable membrane in this set up? EXPLAIN why it is selective!
The filter paper because it just allowed the vapors from the ammonia to get through it but did not allow anything else such as ammonia through it.
3. Is ammonia an acid, a base or neutral? Support your answer. (HINT: look back at our lab background info carefully)
Ammonia is a base because the notes say that the chemical indicator in the gelatin cell only turns pink when it comes in contact with a base.
4. Insert the 3 Red Blood Cell pictures (RBC1, 2 & 3). Under each picture label the solution that the cell is sitting in as hypertonic, hypotonic or isotonic. ALSO, state whether the water moved into the cell, out of the cell or back & forth.
RBC1.png
Hypertonic-out of the cell
RBC2.png
Hypotonic-into the cell
RBC3.png
Isotonic-back and forth

Reflection
In this lab we tested diffusion in a gelatin cell. To do this we put a gelatin cell where it would come in contact with ammonia vapors. Secretly the gelatin cell contained a chemical indicator that made it turn purple when it came in contact with ammonia vapors. We then documented the progress of the ammonia vapors over a period of ten minutes. We did this lab to be able to better visualize diffusion in real life. In this activity we learned several things; first of all ammonia is base, we also learned filter paper does not stop ammonia vapors. In contrast with the gelatin experiment the gummy bear experiment showed the process of osmosis not diffusion. Also the gummy bear got larger and retained its original color. The gelatin cell on the other hand stayed the same size but changed color. However, these two experiments were similar in several ways for example they both show how passive transport works in a large 'cell' that we can witness it first hand.